Persicaria tinctoria is a species of flowering plant in the buckwheat family. Common names include Chinese indigo and "Japanese indigo."[2][3] It is native to Eastern Europe and Asia.The leaves were a source of indigo dye. It was already in use in the Western Zhou period (ca. 1045-771 B.C.), and was the most important blue dye in East Asia until the arrival of Indigofera from the south.
Appl Biochem Biotechnol. 2012 Aug;167(7):1986-2004. doi: 10.1007/s12010-012-9723-7. Epub 2012 May 29.
Chemical composition, antioxidant and anticancer effects of the seeds and leaves of indigo (Polygonum tinctorium Ait.) plant.
Jang HG1, Heo BG, Park YS, Namiesnik J, Barasch D, Katrich E, Vearasilp K, Trakhtenberg S, Gorinstein S.
Seeds and leaves of indigo (Polygonum tinctorium Ait.) plant were investigated and compared with another medicinal plant named prolipid for their properties such as chemical composition, antioxidant, and anticancer effects by Fourier transform infrared, three-dimensional fluorescence spectroscopy, and electrospray ionization-MS in negative mode. It was found that polyphenols, flavonoids, and flavanols were significantly higher in prolipid (P<0.05), following by indigo mature leaves, immature leaves, and seeds. Methanol extract of mature indigo leaves in comparison with the ethyl acetate extract showed higher inhibition of proliferation. The interaction between polyphenol extracts of indigo mature leaves and BSA showed that indigo has a strong ability, as other widely used medicinal plants, to quench the intrinsic fluorescence of BSA by forming complexes. In conclusion, indigo mature leaves were compared with prolipid. High content of bioactive compounds, antioxidant, fluorescence, and antiproliferative properties of indigo justifies the use of this plant as a medicinal plant and a new source of antioxidants.
Extract of the leaves of the indigo plant (Polygonum tinctorium Lour.) was examined for antimicrobial activity against oral pathogenic bacteria. The indigo plant extract at concentrations of 1.74–3.48 mg/ml inhibited the growth of oral cariogenic and periodontopathic bacteria. Tryptanthrin, one of the components of indigo plant extract, showed the strongest antimicrobial effect at doses ranging from 3.13–25 μg/ml, indicating that this compound is one of the active ingredients. Furthermore, treatment of periodontopathic bacteria Porphyromonas gingivalis and Prevotella intermedia with the indigo plant extract for 3–8 h resulted in death of the bacteria in a dose-dependent manner, whereas Streptococcus mutans was not affected by the extract. Thus, for its antimicrobial and bactericidal effects on oral pathogens, indigo plant extract would provide useful material for preventing and treating periodontal diseases and dental caries.
References
1.
Lindquest B, Emilson CG (1991) Dental location of Streptococcus mutans andStreptococcus sobrinus in humans harboring both species. Caries Res 25:146–152PubMedGoogle Scholar
2.
Schupbach P, Osterwalder V, Guggenheim B (1995) Human roots caries: microbiota in plaque covering sound, carious and arrested carious root surfaces. Caries Res 29:382–395PubMedGoogle Scholar
3.
Marsh PD (1994) Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res 8:263–271PubMedGoogle Scholar
4.
Slots J (1999) Update on Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in human periodontal disease. J Int Acad Periodontal 1:121–126Google Scholar
5.
Petit MD, van Steenbergen TJ, Timmerman MF, de Graaff J, van de Velden U (1994) Prevalence of periodontitis and suspected periodontal pathogens in families of adult periodontitis patients. J Clin Periodontol 21:76–85PubMedGoogle Scholar
6.
Tatefuji T, Aga M, Kunikata T, Ikeda M, Kurimoto M (1999) Antiviral effect of Polygonum tinctorium Lour. extracts on virus-infected cells. Nat Med 53:287–301Google Scholar
7.
Inoue S, Takayama S, Ushio S, Iwaki K, Ohashi K, Masaki N, Fukuda S, Ikeda M, Kurimoto M (2000) Improvement of high fat diet-induced hyperlipidemia by Polygonum tinctoriumLour (in Japanese). Nat Med 54:261–264Google Scholar
8.
Kimoto T, Yamamoto Y, Hino K, Koya S, Aga H, Hashimoto T, Hanaya T, Arai S, Ikeda M, Fukuda S, Kurimoto M (1999) Cytotoxic effects of substances in indigo plant (Polygonum tinctorium Lour.) on malignant tumor cells (in Japanese). Nat Med 53:72–79Google Scholar
9.
Kimoto T, Hino K, Koya-Miyata S, Yamamoto Y, Takeuchi M, Nishizaki Y, Micallef MJ, Ushio S, Iwaki K, Ikeda M, Kurimoto M (2001) Cell differentiation and apoptosis of monocytic and promyelocytic leukemia cells (U-937 and HL-60) by tryptanthrin, an active ingredient of Polygonum tinctorium Lour. Pathol Int 52:315–325CrossRefGoogle Scholar
10.
Koya-Miyata S, Kimoto T, Micallef MJ, Hino K, Taniguchi M, Ushio S, Iwaki K, Ikeda M, Kurimoto M (2001) Prevention of azoxymethane-induced intestinal tumors by a crude ethyl acetate-extract and tryptanthrin extracted from Polygonum tinctorium Lour. Anticancer Res 21:3295–3300PubMedGoogle Scholar
11.
Kimoto T, Koya S, Hino K, Yamamoto Y, Aga H, Hashimoto T, Masaki N, Mukai N, Hanaya T, Arai S, Ikeda M, Fukuda S, Kurimoto M (1999) Protection by indigo plant (Polygonum tinctorium Lour.) against renal oxidative damage in mice treated with ferric nitrilotriacetate (in Japanese). Nat Med 53:291–296Google Scholar
12.
Ishihara T, Kohno K, Ushio S, Iwaki K, Ikeda M, Kurimoto M (2000) Tryptanthrin inhibits nitric oxide and prostaglandin E2 synthesis by murine macrophages. Eur J Pharm 407:197–204CrossRefGoogle Scholar
13.
Ishihara T, Okura T, Kohno K Tanimoto T, Ikegami H, Kurimoto M (2000) Polygonum tinctorium extract suppresses nitric oxide production by activated macrophages through inhibiting inducible nitric oxide synthase expression. J Ethnopharmacol 72:141–150CrossRefPubMedGoogle Scholar
14.
Micallef MJ, Iwaki K, Ishihara T, Ushio S, Aga M, Kunikata T, Koya-Miyata S, Kimoto T, Ikeda M, Kurimoto M (2002) The natural plant product tryptanthrin ameliorates dextran sodium sulfate-induced colitis in mice. Int Immunopharmacol 2:565–578CrossRefPubMedGoogle Scholar
15.
Hashimoto T, Aga H, Chaen H, Fukuda S, Kurimoto M (1999) Isolation and identification of anti-Helicobacter pylori compounds from Polygonum tinctorium Lour. Nat Med 53:27–31Google Scholar
16.
Kataoka M, Hirata K, Kunikata T, Ushio S, Iwaki K, Ohashi K, Ikeda M, Kurimoto M (2001) Antibacterial action of tryptanthrin and kaempferol, isolated from indigo plant (Polygonum tinctorium Lour.), against Helicobacter pylori-infected Mongolian gerbils. J Gastroenterol 36:5–9CrossRefPubMedGoogle Scholar