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Lecturer: Ying-Ning Ho (何攖寧)
Email: ynho@mail.ntou.edu.tw
Phone: (02) 24622192 ＃ 5307
Course ID: M34014MW
Credits: 3
Course Prerequisites: Marine microbiology
Course Description and Goals:
As an island country, Taiwan is rich in marine microbial resources. In the development of microbial resources, there are often opportunities to discover marine bacterial species. However, the description and identification of bacterial species require a series of tests and procedures. We hope that students can learn how to identify bacterial species and have the opportunity to do related researches of marine bacteria.
Through this course, students can learn, 1. Background knowledge of physiological and biochemical testing of marine bacteria, and explore the classification and identification of marine bacteria from carbon source utilization, enzyme activity, growth factors, and environmental factors. 2. Learn how to define a new strain and the various tests required to define a new strain (fatty acid composition, genome comparison). 3. Understand the relevant procedures such as international bacteria library and bacterial strain deposit (domestic and foreign deposit).
Schedule Type: Teaching and group discussion
Course Schedule (subject to change):
Part 1: Research on physiological and biochemical functions of marine bacteria
Week 1: Introduction of marine microbial ecology
Week 2: Introduction of marine bacteria screening media
Week 3: Classification and identification of marine bacterial colony morphology and appearance characterization
Week 4: Isolation, purification and preservation of marine bacteria
Week 5: Methods for analyzing physiological and biochemical characteristics of marine bacteria
Week 6: Marine bacterial enzyme activity assay
Week 7: The only carbon source utilization test method for marine bacteria
Week 8: Analysis of chemical characteristics of marine bacterial cell composition
Week 9: Midterm exam week
Part 2: The International strain resource center and the bacterial whole genome sequencing of bacteria
Week 10: Introduction of the new bacteria identification standard of the international bacterial culture collections
Week 11: 16S rRNA alignment and whole genome sequencing in marine bacteria
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