Post date: Aug 14, 2014 3:18:2 PM
For example, pout prepared nutrient agar into a flask or media bottle and cap loosely with a lid or aluminum foil. (Tighten the lid for storage only after the agar solidifying once autoclaved and cooled.)
Add your name and information to the sign-up sheet in the autoclave room. Set the autoclave to 121 C. Also set the sterilization time for 15 min; there is no dry time. Turn the autoclave off when done and wait until the psi reaches 0 before opening the door (this could take up to 30 min.).