GBS library prep protocol
Notes:
Keep track of which EcoRI barcode adapters used
Procedure can be broken into two parts: (1) Restriction and ligation reactions; (2) PCR amplification
After part one the plate can be left in the thermalcycle since it stays at a refrigerated temp. (~ 4 Celsius) but must be put in the fridge the next morning
After the first round of PCR, need to quickly (but not hastily) add reagents for extra PCR step
Fragment size: 280-480 bp
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