Post date: Jan 11, 2018 10:3:41 PM
I ordered new copies of the MseI-1, MseI-2, Illumina_pcr-1, and Illumina_pcr-2 oligos from Eurofnis. They now have an option for NGS Grade HPSF purification, which I used. We received much higher amounts of oligs, 1580.7-2819.8 ug of DNA, such that dillutions for 100 uM ranged from 1350-2203 ul. In fact, these were so high (the volume would not have fit in the tubes), that I diluted them to 200 uM instead. The new oligos have this concentration label, and say NGS Grade HPSF (and have an *) on the tube.