Post date: Jun 23, 2014 4:55:49 PM
I used Qiagen's HMW DNA extraction kit to isolate DNA from fifteen additional samples for whole genome sequencing. This was because the first round of samples failed QC (the DNA was degraded). This could be because they sat in the fridge before being sent out (?).
The new set were extracted as previously described, but were immediately frozen at -80 and shipped on dry ice (though they took four days to arrive and the dry ice almost certainly sublimated on the way there).
Here are the samples (gel image here; 1% gel, 10 ul produce, 1.5 hrs. @ 130 volts):