Post date: Jun 07, 2017 8:38:50 PM
Sam is extracting Lycaeides DNA from 2 new plates. These are from DBS and nearby sites (and BST) and are for a project focused on the Dubois hybrid zone (with an initial emphasis on thinking about diapause/phenology). As usual, we are using Qiagen's DNeasy 96 Blood and Tissue kit. I am then preparing GBS libraries using our standard approach. Fragments between 300 and 450 bp will size selected on the BluePippin (this is the same range we used for the last bunch of Lycaeides data) Here are the dates and plates,
Note, these libraries were prepared with fresh aliquots of the barcodes (done by Carol).