GBS library preps: C. maculatus new lentil lines

Post date: Dec 01, 2015 11:39:48 PM

Caroline is prepping libraries for cal-009, cal-010, cal-011, cal-012, cal-013, cal-014, cal-015, cal-016, cal-017.

Finished libraries with barcode plate information can be found here.

Aaron purified the libraries using the BluePippin (250 to 350 bp). Here are the results.

Based on his comments,

"As I was running them, #15 seemed to have something in it that affected the migration because the markers came up at a different time than the other samples. There seems to be a lot more DNA in that sample, and the size is smaller than the others and smaller than what was expected. Otherwise, the others all look good."

I decided to re-prep cal-015. Note however, that the new version uses CCN's primers (see here).

Here is the final information with library numbers. Note that I didn't end up using cal-009 or cal-010.