Post date: Apr 16, 2018 8:9:43 PM
We reared caterpillars from eggs collected in 2017 to obtain CHC data and map CHC variation. Tara ran this experiment. We reared the caterpillars for 14 days at 14:10 (light:dark) with ~20% RH (we used a dehumider). This is thus potentially an interesting contrast with last years data where humidity was higher (plants were in the incubator, which kept RH up). We reared them on alfalfa grown from seed in our incubator (and some in Mary's greenhouse).
Here is the data sheet with weights and notes. CHC_caterpillar_2018_weights
We send the caterpillars (killed by freezing at -80C and placed in glass vials) to Gary at UNR for CHC extractions (in hexane). He is then sending them back for us to do the molecular work.
We received the samples. Tara extracted DNA using the standard Qiagen DNeasy kit. She ran a few check gels to make sure we were getting DNA from the samples and things generally looked good. She then prepared GBS libraries using our standard protocol.
We had Aaron Thomas run the samples on the BluePippin and BioAnalyzer. We purified 300-450 bp with the BluePippin. Here are the BioAnalyzer reports.
We had this sequenced as a single run on a NextSeq here at USU. We ran the high output mode with 150 cycles (1x150).