Post date: Aug 26, 2014 3:1:54 PM
I sent the three AF samples (AF1, AF2, and AF3) to BGI for whole genome sequencing. These will be used to generate and sequence a shotgun library (180 bp insert), a 3 Kb insert library and a 5 Kb insert library. The shotgun library will be sequenced on one lane and the two larger insert libraries will be sequenced on a second lane (HiSeq 2000, 100 bp PE reads). As these are all from the same individual, I told BGI to combine and use them however they wanted to construct the libraries.