Post date: May 13, 2014 7:0:41 PM
I completed two rounds of extractions of high-molecular weight DNA with the MagAtrract HMW kit from 20 Lycaeides. I hope to use this for whole genome sequencing, with three libraries for each of five entities: shotgun, nextera 3kb, nextera 5 kb. I followed this protocol, with the following modifications. Shaking occurred at 500 rpm (machine limitation), and the final elution was in 150 ul of nuclease free water (provided with the kit). I am more confident in the quality of the second set of extractions, but I need to perform QC on all 20 samples. Here is the sample information (these are all females):
Ning-Ling ran these samples on the NanoDrop and I added the 260/280 and DNA concentrations to the table above. They look pretty good.