În comparație cu procesul convențional de electrofilare (electrospinning), tehnica E-jetting oferă o bună controlabilitate a orientării fibrelor într-un mod precis, demonstrând fezabilitatea ridicată a construirii unei structuri 3D complicate cu fibre fine pentru diverse aplicații în fabricarea de senzori, dispozitive optice și ingineria țesuturilor. Studiul de imprimare a celulelor a demonstrat că imprimarea piezoelectrică DoD este capabilă să livreze cu succes celule fibroblaste de șobolan L929 prin duze de până la 36 μm. Nu a existat o moarte celulară semnificativă la distribuirea celulelor prin duza de 81 μm și 119 μm, ratele medii de supraviețuire reducându-se doar de la 98% la 85%. Acest lucru este în acord cu studiul existent raportat (Saunders et al. 2008), în care o imprimantă comercială a fost folosită pentru a imprima celule fibroblaste umane.

Referințe

Barron JA, Krizman DB, Ringeisen BR (2005) Laser printing of single cells: statistical analysis, cell viability, and stress. Ann Biomed Eng 33(2):121–130

Chang SC, Liu J et al (1999) Multicolor organic light-emitting diodes processed by hybrid inkjet printing. Adv Mater 11(9):734–737

Chang C, Limkrailassiri K, Lin LW (2008a) Continuous near-field electrospinning for large area deposition of orderly nanofiber patterns. Appl Phys Lett 93:2008

Chang R, Nam J, Sun W (2008b) Direct cell writing of 3d microorgan for in vitro pharmacokinetic model. Tissue Eng Part C Methods 14:157–166

Chang R, Nam J, Sun W (2008c) Effects of dispensing pressure and nozzle diameter on cell survival from solid freeform fabrication-based direct cell writing. Tissue Eng Part A 14(1):41–48

Chen CY, Barron JA, Ringeisen BR (2006) Cell patterning without chemical surface modification: cell-cell interactions between printed bovine aortic endothelial cells (BAEC) on a homogeneous cell-adherent hydrogel. Appl Surf Sci 252(24):8641–8645

Deegan RD, Bakajin O et al (1997) Capillary flow as the cause of ring stains from dried liquid drops. Nature 389:827–829

Doshi J, Reneker DH (1995) Electrospinning process and applications of electrospun fibers. J Electrostat 35:151–160

Formhals A (1934) Process and apparatus for preparing artificial threads. US Patent 1975504

Heinzl J, Hertz CH (1985) Ink-jet printing. Adv Electron Electron Phys 65:91–171

Ikegawa M, Azuma H (2004) Droplet behaviors on substrates in thin-film formation using ink-jet printing. JSME Int J Ser B: Fluid Therm Eng 47(3):490–496

Jaworek A, Sobczyk AT (2008) Electrospraying route to nanotechnology: an overview. J Electrostat 66:197–219

Kyocera Corporation (2009) Website cited: http://global.kyocera.com/news/2009/0903_kdos.html

Li EQ (2010) The generation and experimental study of microscale droplets in drop- on-demand inject printing. Ph.D. thesis, National University of Singapore

Liao IC, Chew SY, Leong KW (2006) Aligned core-shell nanofibers delivering bioactive proteins. Nanomedicine 1:465–471

Nakamura M, Kobayashi A et al (2005) Biocompatible inkjet printing technique for designed seeding of individual living cells. Tissue Eng 11(11–12):1658–1666

Nakashima Y, Hayashi K, Inadome T et al (1997) Hydroxyapatite coating on titanium arc sprayed titanium implants. J Biomed Mater Res 35:287–298

Pham QP, Sharma U, Mikos AG (2006) Electrospinning of polymeric nanofibers for tissue engineering applications: a review. Tissue Eng 12:1197–1211

Rayleigh L (1878) On the instability of jets. Proc Lond Math Soc 10(4):4–13

Saunders RE, Gough JE, Derby B (2008) Delivery of human fibroblast cells by piezoelectric dropon-demand inkjet printing. Biomaterials 29(2):193–203

Singh M, Haverinen HM, Dhagat P, Jabbour GE (2010) Inkjet printing – progress and its applications. Adv Mater 22:673–685

Sun DH, Chang C, Li S, Lin LW (2006) Near-field electrospinning. Nano Lett 6:839–842

Sun J, Chang L et al. (2012) Bio-inspired organic composite coatings for implants via microdispensing technique. Adv Mater Process 662–672, 10th Asia-Pacific conference on materials processing, Jinan, China, 14–17 June 2012

Takahashi S, Tomikawa Y (2003) A study of handling of living mammalian cells using a piezoelectric dispensing head. Japan J Appl Phys 42:3098–3101

Thirumalpathy PVK, Leroy MJ (2011) Experimental investigation on the operating variables of a near-field electrospinning process via a response surface methodology. J Manuf Proc 13(2011):104–112

Walter D, Niles P, Coassin J (2005) Piezo- and solenoid valve-based liquid dispensing for miniaturized assays. ASSAY Drug Dev Technol 3:189–202

Wang XY, Drew C, Lee SH et al (2002) Electrospun nanofibrous membranes for highly sensitive optical sensors. Nano Lett 2:1273–1275

Xu T, Petridou S et al (2004) Construction of high-density bacterial colony arrays and patterns by the ink-jet method. Biotechnol Bioeng 85(1):29–33

Xu T, Jin J et al (2005) Inkjet printing of viable mammalian cells. Biomaterials 26(1):93–99

Xu T, Gregory CA et al (2006) Viability and electrophysiology of neural cell structures generated by the inkjet printing method. Biomaterials 27(19):3580–3588