Biology PAG 6.3

• Pag 6.3 Chromatography :          HazCard :  85A,42, 45A

•  Thin layer silica gel chromatography plate

• Chromatography kit 4A2

•  Leaves (spinach)

• Pestle and mortar

• sand to speed up the mash up of spinach

• Pencil

• Ruler

• Goggles 

• gloves (always with organic chemical)

• Propanone (extraction solvent) (Acetone in test tube) in dropper bottle              

(propanone = Acetone   propanone is (chemistry) the systematic name of the simplest ketone with formula C3H6O, usually called acetone while acetone is (organic compound) a colourless, volatile, flammable liquid ketone, (CH3)2CO, used as a solvent.)

• Chromatography solvent (1 part propanone, 1 part ethoxyethane, 2 part petroleum ether 80 oC)

• Small filter paper

• Small filter funnel

• white tile

• Test tube  (Pigment) use small beaker instead 

• Micro-pipette and disposable tip

• Chromatography tank (Chromatography kit) in fume unit, containing chromatography solvent to a depth of 0.5 cm

• (Spatula)