2.1 Biomedical test

2.1 part 1-2-3 Biochemical test :                HazCard :

Part 1 :   Starch          HazCard :

• 3 X Test tubes Permanent markers

• Pipettes or syringes

• 1 % Starch solution (5cc)

• 10 % Glucose solution (5cc)

• Distilled water (5cc)

• Iodine dropper bottle (5 drops in each test tubes)

Part 2 :   Proteins      HazCard :

• 3 X Test tubes, Permanent markers

• Pipettes

• 1 % Protein solution (Albumen or Casein) (5cc)

• 10 % Glucose solution (5cc)

• Distilled Water (5cc)

• Biuret (5cc in each test tube)

Part 3:    Reducing sugar        HazCard :                Gaz ON

• 6 X Test tubes, Permanent markers

• Pipettes or syringes

• 10 % Glucose (5cc)

• 10 % Sucrose, Maltose, Fructose, Lactose solution (5CC each)

• Distilled Water 5cc

• Benedict solution (5cc in each test tube)

• Water bath 85 degrees (or Bunsen...)

• Heat proof Gloves 

• Test tubes Holder

• Timers

Part 4 :  Non Reducing Sugar     HazCard :

• 2 X test Tubes, rack, Permanent markers

• Pipettes or syringes

• Distilled water 5cc

• 10% Sucrose solution (5cc)

• Dilute HCl 2.0 M (2 drops in each test tube)

• Water bath 85 degrees (Or bunsen...)

• Spatula

• Sodium Hydrogen carbonate (Small amount)

• Benedict solution (5 cc in each test tube)

Part 5 :   Emulsion for lipids  HazCard : 

• 2 X Test tubes, Permanent marker pens

• pipettes or syringes

• Vegetable oil (2 drops)

• Ethanol (5 cc in each test tubes)

• Distilled water (5cc in each test tube)

Part 6 :     Unknown solution   HazCard : 

• 3 X Unknown combination solution 

  • • Solution X : 1g Sucrose, 1 g Albumin dissolve in 100cm3 Distilled water

    • Solution Y : 1g Sucrose dissolve in 100cm3 Distilled water

    • Solution Z : 1g Glucose, 1 g Starch dissolved in 100cm3 Distilled water

• Iodine

• Biuret

• Benedict

• Ethanol

• HCl 2.0 M

• Sodium Hydrogen  Carbonate