Enzyme preparation for experiments

Here is a recipe that is suitable to prepare 100 mL of a 0.5% w/v solution of trypsin, pepsin or amylase using distilled water.

Wear PPE: safety glasses, gloves, laboratory coat, face mask or work in a fume cupboard that is not turned on to minimise exposure to dust or aerosols. If working outside a fume cupboard, make sure you are in a draft-free area.

• Weigh out 0.5g of the enzyme.

• Add to 80mL of distilled water at room temperature in a beaker.

• Stir gently to dissolve.

• Adjust to a final volume of 100ml.

• Store at 4° C (fridge) for a short period of time or on ice during use.

• Do not heat or allow the solution to froth, as this will denature the enzyme.