Core practical 1: Investigate a factor affecting the initial rate of reaction

Equipment : HazCard :

•  skimmed milk powder suspension (2%) 

•  standard protease (trypsin) solution (1%) IRRITANT 

•  6 test tubes and holder  

•  stop clock  

•  two 5 cm3 pipettes (or syringes/measuring cylinders) 

•  eye protection  

•  access to colorimeter (see diagram) (or light meter with datalogger) 

•  2 cuvettes 

•  distilled water