SREL Reprint #3416

Development, characterization, and utility of 13 polymorphic microsatellite loci in Praticolella (Gastropoda: Polygyridae) species from South Texas, U.S.A.

Russell L. Minton1, Rochelle R. Beasley2, Stacey L. Lance2, and Kathryn E. Perez3

1Department of Biological and Environmental Sciences, University of Houston Clear Lake,
2700 Bay Area Boulevard MC 39, Houston, Texas 77058, USA
2Savannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802, USA
3Department of Biology, University of Texas Rio Grande Valley, 1201 West University Drive,
Edinburg, Texas 78539, USA

Abstract: We developed and characterized 13 polymorphic microsatellite loci in South Texas species of Praticolella von Martens, 1892 (Gastropoda: Polygyridae), some of which have restricted distributions and can be difficult to identify using traditional shell characters. To make this genomic tool more broadly useful in these species, instead of characterizing loci in many individuals of a single species, we tested them across five populations of four different species. Twelve loci amplified in all four species tested, and eleven loci showed deviations from Hardy-Weinberg equilibrium. These loci will be valuable in assessing species identity and population genetic connectivity among South Texas Praticolella.

Keywords: Praticolella salina, population genetics, Fst

SREL Reprint #3416

Minton, R. L., R. R. Beasley, S. L. Lance, and K. E. Perez. 2017. Development, characterization, and utility of 13 polymorphic microsatellite loci in Praticolella (Gastropoda: Polygyridae) species from South Texas, U.S.A. American Malacological Bulletin 35(2): 158-162.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).