SREL Reprint #3188

Development and characterization of 12 microsatellite loci for the Dwarf Salamander, Eurycea quadridigitata

Anna M. Mckee1,2, Stacey L. Lance3, Kenneth L. Jones4, Cris Hagen3, and Travis C. Glenn2,4

1Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA
2Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA
3Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
4Georgia Genomics Facility, University of Georgia, Athens, GA 30602, USA

Abstract: We isolated and characterized 12 microsatellite loci for the Dwarf Salamander, Eurycea quadridigitata. Loci were screened in 30 individuals of E. quadridigitata. The number of alleles per locus ranged from 2 to 9, observed heterozygosity ranged from 0.2 to 0.9, and the probability of identity values ranged from 0.034 to 0.689. These new loci can be used to study population genetic structure and potentially help determine cryptic species divergence.

Keywords: Dwarf Salamander, Eurycea, Microsatellite, PCR primers, SSR, STR

SREL Reprint #3188

McKee, A. M., S. L. Lance, K. L. Jones, C. Hagen, and T. C. Glenn. 2011. Development and characterization of 12 microsatellite loci for the Dwarf Salamander, Eurycea quadridigitata. Conservation Genetic Resources 2011(3): 633-635.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).