SREL Reprint #3139

Five hundred microsatellite loci for Peromyscus

Jesse N. Weber1, Maureen B. Peters2, Olga V. Tsyusko2,3, Catherine R. Linnen1, Cris Hagen2, Nancy A. Schable2, Tracey D. Tuberville2, Anna M. McKee2, Stacey L. Lance2,4, Kenneth L. Jones4, Heidi S. Fisher1, Michael J. Dewey5, Hopi E. Hoekstra1, and Travis C. Glenn2,4

1Department of Organismic and Evolutionary Biology and The Museum of Comparative Zoology,
Harvard University, Cambridge, MA 02138, USA
2Savannah River Ecology Laboratory, Drawer E, Aiken, SC 29802, USA
3Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546, USA
4Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA
5Peromyscus Genetic Stock Center, Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA

Abstract: Mice of the genus Peromyscus, including several endangered subspecies, occur throughout North America and have been important models for conservation research. We describe 526 primer pairs that amplify microsatellite DNA loci for Peromyscus maniculatus bairdii, 467 of which also amplify in Peromyscus polionotus subgriseus. For 12 of these loci, we report diversity data from a natural population. These markers will be an important resource for future genomic studies of Peromyscus evolution and mammalian conservation.

Keywords: Microsatellite, Peromyscus maniculatus, Peromyscus polionotus, SSR, STR, PCR primers.

SREL Reprint #3139

Weber, J. N., M. B. Peters, O. V. Tsyusko, C. R. Linnen, C. Hagen, N. A. Schable, T. D. Tuberville, A. M. McKee, S. L. Lance, K. L. Jones, H. S. Fisher, M. J. Dewey, H. E. Hoekstra, and T. C. Glenn. 2010. Five hundred microsatellite loci for Peromyscus. Conservation Genetics 2010(11): 1243-1246.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).