SREL Reprint #2841

Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)

Brant C. Faircloth1, Allison Reid1, Teresa Valentine1, Soo Hyung Eo1, Theron M. Terhune1, Travis C. Glenn2,3, William E. Palmer4, Campbell Joseph Nairn1, and John P. Carroll1

1D. B. Warnell School of Forest Resources, University of Georgia, Athens, GA, 30602, USA
2Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, SC 29802, USA
3Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA
4Tall Timbers Research Station, 13093 Henry Beadel Drive, Tallahassee, FL 32312, USA

Abstract: We describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rutus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range four to 10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78).

Keywords: bobcat, dinucleotide repeats, Lynx rufus, microsatellites, primers, SSRs, tetranucleotide repeats, trinucleotide repeats

SREL Reprint #2841

Faircloth, B. C., A. Reid, T. Valentine, S. H. Eo, T. M. Terhune, T. C. Glenn, W. E. Palmer, C. J. Nairn, and J. P. Carroll. 2005. Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus). Molecular Ecology Notes 5:387-389.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).