SREL Reprint #3315

Genus-wide microsatellite primers for the goldenrods (Solidago: Asteraceae)

James B. Beck1, John C. Semple2, Justin M. Brull1, Stacey L. Lance3, Mai M. Phillips4, Sara B. Hoot5,
and Gretchen A. Meyer6

1Department of Biological Sciences, Wichita State University,
537 Hubbard Hall, Wichita, Kansas 67260 USA
2Department of Biology, University of Waterloo, Waterloo, Ontario NL2 3G1, Canada
3Savannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802 USA
4Conservation and Environmental Science Program, University of Wisconsin–Milwaukee,
3209 N. Maryland Ave., Milwaukee, Wisconsin 53201 USA
5Department of Biological Sciences, University of Wisconsin–Milwaukee,
3209 N. Maryland Ave., Milwaukee, Wisconsin 53201 USA
6Field Station, University of Wisconsin–Milwaukee, 3095 Blue Goose Road,
Saukville, Wisconsin 53080 USA

Premise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus Solidago.
Methods and Results: Illumina sequencing of a shotgun library from S. gigantea identified ca. 1900 putative single-copy loci. Fourteen loci were subsequently shown to be amplifiable, single-copy, and variable in a broad range of Solidago species.
Conclusions: The utility of these markers both across the genus and in herbarium specimens of a wide age range will facilitate numerous inter- and intraspecific studies in the ca. 120 Solidago species.

Keywords: Asteraceae; Illumina sequencing; polyploidy; simple sequence repeat (SSR) markers; Solidago.

SREL Reprint #3315

Beck, J. B., J. C. Semple, J. M. Brull, S. L. Lance, M. M. Phillips, S. B. Hoot, and G.A. Meyer. 2014. Genus-wide microsatellite primers for the goldenrods (Solidago: Asteraceae). Applications in Plant Sciences 2(4): 1300093.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).