SREL Reprint #3172

Development and characterization of 18 microsatellite loci for the Southern Leopard Frog, Rana sphenocephala

Anna M. McKee1, Stacey L. Lance2, Kenneth L. Jones3, Cris Hagen2, and Travis C. Glenn3,4

1Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA
2Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
3Georgia Genomics Facility, University of Georgia, Athens, GA 30602, USA
4Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA

Abstract: We isolated and characterized 18 microsatellite loci for the Southern Leopard Frog, Rana sphenocephala. Loci were screened in 30 individuals of R. sphenocephala. The number of alleles per locus ranged from 5 to 21, observed heterozygosity ranged from 0.200 to 0.933, and the probability of identity values ranged from 0.008 to 0.299. These new loci are tools that can be used to study population genetic structure, genetic diversity, and gene flow across varying habitat types and scales.

Keywords: Southern Leopard Frog, Rana, Lithobates, Microsatellite, PCR primers, SSR, STR

SREL Reprint #3172

McKee, A. M., S. L. Lance, K. L. Jones, C. Hagen, and T. C. Glenn. 2011. Development and characterization of 18 microsatellite loci for the Southern Leopard Frog, Rana sphenocephala. Conservation Genetics Resources (2011) 3:267-269.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).