SREL Reprint #3226

Microsatellite development for an endangered riparian inhabitant, Lilaeopsis schaffneriana subsp. recurva (Apiaceae)

Jessica M. Tew1, Stacey L. Lance2, Kenneth L. Jones3, and Shannon D. Fehlberg1

1Desert Botanical Garden, 1201 North Galvin Parkway, Phoenix, Arizona 85008 USA
2Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, South Carolina 29802 USA
3University of Colorado School of Medicine, Aurora, Colorado 80045 USA

Premise of the study: Microsatellite markers were developed and characterized to evaluate genetic diversity and population structure in Lilaeopsis schaffneriana subsp. recurva, an endangered species endemic to wetlands dispersed throughout southeastern Arizona, USA, and northern Sonora, Mexico.
Methods and Results: Eight loci (one of which was monomorphic) were developed and characterized in 48 individuals from two populations. The total number of alleles was 35, ranging from one to 10 per locus. Many of the primers amplified in L. carolinensis, L. chinensis, L. masonii, L. occidentalis, L. schaffneriana subsp. schaffneriana, Oxypolis fendleri, and Eryngium lemmonii.
Conclusions: Development of these novel microsatellite loci will facilitate a deeper understanding of genetic diversity, mode of reproduction, and population structure not only in L. schaffneriana subsp. recurva, but also in apiaceous relatives.

Keywords: Apiaceae; clonal growth; genetic diversity; Lilaeopsis schaffneriana subsp. recurva; microsatellites

SREL Reprint #3226

Tew, J. M., S. L. Lance, K. L. Jones, and S. D. Fehlberg. 2012. Microsatellite development for an endangered riparian inhabitant, Lilaeopsis schaffneriana subsp. recurva (Apiaceae). American Journal of Botany 99(4): e164-e166.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).