SREL Reprint #2395

Lack of demonstratable effects of pollutants on cyt b sequences in wood ducks from a contaminated nuclear reactor cooling pond

Kevin P. Johnson1, Jason Stout2, I. Lehr Brisbin, Jr.3, Robert M. Zink1, and Joanna Burger2

1Department of Ecology, Evolution, and Behavior and Bell Museum of Natural History,
University of Minnesota, St. Paul, Minnesota 55108;
2Department of Ecology, Evolution and Natural Resources, Nelson Biological Laboratories,
Rutgers University, Piscataway, New Jersey and Environmental and Occupational Health
Sciences Institute, Piscataway, New Jersey
3Savannah River Ecology Laboratory, Aiken, South Carolina

Abstract: The effects of low levels of radiation on DNA mutation rates are largely unknown for free-living vertebrates. In this study we investigated the effects of contamination from cooling ponds at a nuclear production facility in South Carolina on the mutation rates in mitochondrial DNA in wood ducks (Aix sponsa). Specifically, we sequenced a 433-bp portion of the cytochrome b gene from 18 female-offspring pairs of wood ducks from contaminated ponds and 2 female-offspring pairs from control ponds. Very low haplotype diversity was observed overall, and no case of mutation between female and offspring could be satisfactorily documented. This suggests that the levels of radioactive contamination in these cooling ponds have little effect on the mutation rate of mitochondrial DNA in these waterfowl and that mitochondrial DNA may not be as sensitive an indicator as previously anticipated. 

SREL Reprint #2395

Johnson, K.P., J. Stout, I.L. Brisbin, Jr., R.M. Zink, and J. Burger. 1999. Lack of demonstratable effects of pollutants on cyt b sequences in wood ducks from a contaminated nuclear reactor cooling pond. Environmental Research Section A 81:146-150.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).