SREL Reprint #3297

Twenty-five novel microsatellite markers for English sole, Parophrys vetulus

Gary A. Winans1, Jon D. Baker2, and Stacey L. Lance3

1NOAA Northwest Fisheries Science Center, Seattle, WA 98112, USA
2Douglas Salmometrics, Mukilteo, WA 98275, USA
3Savannah River Ecology Laboratory, Aiken, SC 29802, USA

Abstract: Although English sole Parophrys vetulus has been used extensively as a bioindicator of anthropogenic pollution in the US Pacific Northwest, little is known about its genetic population structure. We isolated and characterized a total of 25 microsatellite loci from 23 individuals from Whidbey Island, Washington. The number of alleles per locus ranged from 5 to 19, observed heterozygosity ranged 0.091–0.957, and the probability of identity values ranged from 0.009 to 0.157. These new loci will be used to study genetic variability within and among populations in the Salish Sea (Puget Sound and Strait of Georgia) to help monitor effects of near shore urbanization, and establish and monitor marine protected areas.

Keywords: Parophrys vetulus, Microsatellite, Illumina, Pal_finder, SSR, Population markers

SREL Reprint #3297

Winans, G. A., J. D. Baker, and S. L. Lance. 2014. Twenty-five novel microsatellite markers for English sole, Parophrys vetulus. Conservation Genetic Resources 2014(6): 417-419.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).