SREL Reprint #3330

Development and characterization of microsatellite loci for the endangered scrub lupine, Lupinus aridorum (Fabaceae)

Angela Ricono1, Glen Bupp2, Cheryl Peterson2, Schyler O. Nunziata3, Stacey L. Lance3,
and Christin L. Pruett1

1Department of Biological Sciences, Florida Institute of Technology, Melbourne, Florida 32901 USA
2Rare Plant Conservation Program, Bok Tower Gardens, Lake Wales, Florida 33853 USA
3Savannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802 USA

• Premise of the study: Microsatellite primers were developed in scrub lupine (Lupinus aridorum, Fabaceae), an endemic species to Florida that is listed as endangered in the United States, to assess connectivity among populations, identify hybrids, and examine genetic diversity.
• Methods and Results: We isolated and characterized 12 microsatellite loci polymorphic in scrub lupine or in closely related species (i.e., sky-blue lupine [L. diffusus] and Gulf Coast lupine [L. westianus]). Loci showed low to moderate polymorphism, ranging from two to 14 alleles per locus and 0.01 to 0.86 observed heterozygosity.
• Conclusions: These loci are the first developed for Florida species of lupine and will be used to determine differentiation among species and to aid in conservation of the endangered scrub lupine.

Keywords: Fabaceae; Florida; Lupinus; microsatellite; PCR primers

SREL Reprint #3330

Ricono, A., G. Bupp, C. Peterson, S. O. Nunziata, S. L. Lance, and C. L. Pruett. 2015. Development and characterization of microsatellite loci for the endangered scrub lupine, Lupinus aridorum (Fabaceae). Applications in Plant Sciences 3(4): 1-3.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).