SREL Reprint #2725

Microsatellite loci isolated from narrow-leaved cattail Typha augustifolia

Olga V. Tsyusko-Omeltchenko, Nancy A. Schable, Michael H. Smith, and Travis C. Glenn

The University of Georgia, Institute of Ecology, Savannah River Ecology Laboratory,
P.O. Drawer E, Aiken, SC 29802, USA

Abstract: We present 11 dinucleotide micro satellite DNA loci isolated from the narrow-leaved cattail (Typha angustifolia) and describe conditions for their amplification. The PCR primers were tested on at least 20 individuals of Typha angustifolia and T. latifolia from two Ukrainian populations per species. The primers amplify loci with relatively high numbers of alleles (averaging 7.22 and 4.95 alleles per locus in T. angustifolia and T. latifolia, respectively); and polymorphic information content (averaging 0.61 and 0.46 in T. angustifolia and T. latifolia, respectively).

Keywords: dinucleotide repeats, microsatellite, PCR, primer, radiation, Typha angustifolia, Typha latifolia, Ukraine

SREL Reprint #2725

Tsyusko-Omeltchenko, O. V., N. A. Schable, M. H. Smith, and T. C. Glenn. 2003. Microsatellite loci isolated from narrow-leaved cattail Typha angustifolia. Molecular Ecology Notes 3:535-538.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).