SREL Reprint #3270

Development and characterization of thirty novel microsatellite markers for the critically endangered Myanmar Roofed Turtle, Batagur trivittata, and cross-amplification in the Painted River Terrapin, B. borneoensis, and the Southern River Terrapin, B. affinis, using paired-end Illumina shotgun sequencing

Cara N. Love1, Cris Hagen1, Brian D. Horne2, Kenneth L. Jones3, and Stacey L. Lance1

1Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
2Wildlife Conservation Society, 2300 Southern Blvd Bronx, New York, NY 10460, USA
3Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine,
Aurora, Denver, CO 80045, USA

Abstract: We isolated and characterized 30 microsatellite loci from the critically endangered Myanmar Roofed Turtle, Batagur trivittata. Loci were screened in 9 B. trivittata samples and in the congeners the Painted River Terrapin, Batagur borneoensis, with 22 of 30 amplifying, and the Southern River Terrapin , B. affinis, with 15 of 30 amplifying. In the B. trivittata samples, the number of alleles per locus ranged from 3 to 10 and the probability of identity values ranged from 0.031 to 0.354. These new loci will provide tools for captive management and reintroduction programs for B. trivittata and the other five species of Batagur, particularly B. affinis in Cambodia.

Keywords: Batagur, Illumina, Microsatellite, PAL_FINDER, PCR primers, SSR

SREL Reprint #3270

Love, C. N., C. Hagen, B. D. Horne, K. L. Jones, and S. L. Lance. 2013. Development and characterization of thirty novel microsatellite markers for the critically endangered Myanmar Roofed Turtle, Batagur trivittata, and cross-amplification in the Painted River Terrapin, B. borneoensis, and the Southern River Terrapin, B. affinis, using paired-end Illumina shotgun sequencing. Conservation Genetic Resources 5(2): 383-387.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).