SREL Reprint #3158

Development and characterization of ten mcirosatellite loci for the eastern spadefoot toad, Scaphiopus holbrookii

Matthew R. Erickson1, David E. Scott1, Kenneth L. Jones2, Cris Hagen1, and Stacey L. Lance1

1Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
2Georgia Genomics Facility, University of Georgia, Athens, GA 30602, USA

Abstract: We isolated and characterized 10 microsatellite loci from the eastern spadefoot toad, Scaphiopus holbrookii. Loci were screened in 24 individuals from two schools of tadpoles in a single isolated wetland in South Carolina, USA. The number of alleles per locus ranged from 4 to 12, observed heterozygosity ranged from 0.200 to 0.875, and the probability of identity values ranged from 0.043 to 0.298. These new loci provide tools for examining the landscape genetics of a species facing continued destruction of its breeding habitat (small isolated wetlands) as well as fragmentation of upland life zones.

Keywords: Scaphiopus, Spadefoot toad, Microsatellite, PCR primers, SSR, STR

SREL Reprint #3158

Erickson, M. R., D. E. Scott, K. L. Jones, C. Hagen, and S. L. Lance. 2010. Development and characterization of ten mcirosatellite loci for the eastern spadefoot toad, Scaphiopus holbrookii. Conservation Genetics Resources 2010(2): 143-145.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).