SREL Reprint #3233

Development of 24 microsatellite markers for the white nosed coati (Nasua narica) using 454 sequencing

Eduardo Sánchez Garibay1, Adrian Silva-Caballero1, Melina Del Real-Monroy1, Stacey L. Lance2, David Valenzuela-Galván3, and Jorge Ortega1

1Laboratorio de Ictiología y Limnología, Posgrado en Ciencias Quimicobiológicas, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Sto. Tomas, 11340 Mexico, DF, Mexico
2Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA
3Centro de Investigación en Biodiversidad y Conservación, Universidad Autónoma del estado de Morelos (CIByC-UAEM), Av. Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, Mexico

Abstract: The White nosed coati (Nasua narica) it is a highly social carnivore inhabiting mainly Neotropical forests playing an important ecological role. A technique based on 454 sequencing of a library highly enriched for microsatellite repeats was used to develop loci for Nasua narica. Twenty-four polymorphic microsatellites were developed and tested as markers in the target species. All markers were genotyped on 18 different individuals from three distinct locations. We observed medium to low genetic variation across most loci (mean number of alleles per locus = 5). Levels of expected heterozygosity across all markers was fairly low (mean HE = 0.326, range 0.05–0.49).

Keywords: 454 Sequencing, Expected heterozygosity, Nasua narica, Microsatellites

SREL Reprint #3233

Garibay, E. S., A. Silva-Caballero, M. Del Real-Monroy, S. L. Lance, D. Valenzuela-Galván, and J. Ortega. 2012. Development of 24 microsatellite markers for the white nosed coati (Nasua narica) using 454 sequencing. Conservation Genetic Resources 2012(4): 661-663.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).