SREL Reprint #3253

Development and characterization of twenty-two novel microsatellite markers for the mountain whitefish, Prosopium williamsoni and cross-amplification in the round whitefish, P. cylindraceum, using paired-end Illumina shotgun sequencing

Jason O’Bryhim1, Christopher Somers2, Stacey L. Lance1, Monica Yau3, Douglas R. Boreham4,5, Kenneth L. Jones6, and Eric B. Taylor3

1Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA
2Department of Biology, University of Regina, Regina, SK S4S 0A2, Canada
3Department of Zoology, Biodiversity Research Centre and Beaty Biodiversity Museum,
University of British Columbia, Vancouver, BC V6T 1Z4, Canada
4Bruce Power, Integration Department, Tiverton, ON N06 2T0, Canada
5Department of Medical Physics and Applied Radiation Sciences, McMaster University,
Hamilton, ON L8S 4K1, Canada
6Department of Biochemistry and Molecular Genetics, School of Medicine,
University of Colorado, Aurora, CO 80045, USA

Abstract: We isolated and characterized a total of 22 microsatellite loci in Prosopium williamsoni. Loci were screened in 24 individuals from across a portion of its range in the Peace River area of northeastern British Columbia. The number of alleles per locus ranged from 3 to 19, observed heterozygosity ranged from 0.111 to 0.950, and the probability of identity values ranged from 0.013 to 0.604. These new loci will be used for conducting investigations into the genetic structure and diversity of extant populations of this important forage fish. The loci were also screened in the congener P. cylindraceum, which is of interest as an indicator species in the Great Lakes region of North America; 15 of the 22 loci produced scorable PCR products in the round whitefish.

Keywords: Prosopium, Microsatellite, PAL_FINDER, PCR primers, SSR

SREL Reprint #3253

O'Bryhim, J., C. Somers, S. L. Lance, M. Yau, D. R. Boreham, K. L. Jones, and E. B. Taylor. 2013. Development and characterization of twenty-two novel microsatellite markers for the mountain whitefish, Prosopium williamsoni and cross-amplification in the round whitefish, P. cylindraceum, using paired-end Illumina shotgun sequencing. Conservation Genetic Resources 5(1):89-91.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).