SREL Reprint #3173

Ten novel microsatellite markers for the western mosquitofish Gambusia affinis

K. M. Purcell1, S. L. Lance2, K. L. Jones3, and C. A. Stockwell1

1Department of Biological Sciences, North Dakota State University, NDSU Dept. 2715,
218 Stevens Hall, P.O. Box 6050, Fargo, ND 58108-6050, USA
2Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
3Georgia Genomics Facility, University of Georgia, Athens, GA 30602, USA

Abstract: We isolated and characterized 10 microsatellite loci from the western mosquitofish, Gambusia affinis. Loci were screened in 30 individuals from a single location from their native range in coastal Texas. The number of alleles per locus ranged from 4 to 25, observed heterozygosity ranged from 0.40 to 0.93, and the probability of identity values from 6.0 × 10-3 to 2.7 × 10-1, with an overall PI for all loci of 1.3 × 10-13. These new loci provide tools for examining the genetic diversity, structure, and pedigree G. affinis populations.

Keywords: Gambusia, Mosquitofish, Microsatellite, SSR, STR, Poeciliidae

SREL Reprint #3173

Purcell, K. M., S. L. Lance, K. L. Jones, and C. A. Stockwell. 2011. Ten novel microsatellite markers for the western mosquitofish Gambusia affinis. Conservation Genetics Resources (2011) 3:361-363.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).