SREL Reprint #2669

Tetranucleotide micro satellite DNA loci from the dollar sunfish (Lepomis marginatus)

Nancy A. Schable1,2, Robert U. Fischer2, and Travis C. Glenn1

1Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, SC 29802, USA
2Department of Biological Sciences, Eastern Illinois University, Charleston, IL 61920, USA

Abstract: We describe polymerase chain reaction (PCR) primers and conditions to amplify one dinucleotide and eight tetranucleotide microsatellite DNA loci isolated from the dollar sunfish (Lepomis marginatus). The PCR primers were tested on 20 or more individuals from five populations. The dollar sunfish microsatellite primers developed yielded a high number of alleles (4 to 14 per locus), and high observed heterozygosities (0.500-0.857).

Keywords: dinucleotide repeats, Lepomis marginatus, microsatellite, PCR, primer, SSR, tetranucleotide repeats

SREL Reprint #2669

Schable, N. A., R. U. Fischer, and T. C. Glenn. 2002. Tetranucleotide microsatellite DNA loci from the dollar sunfish (Lepomis marginatus). Molecular Ecology Notes 2:509-511.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).