SREL Reprint #3171

Development and characterization of ten polymorphic microsatellite loci in the yellowtail flounder (Limanda ferruginea)

Jonathan S. Breton1, Kenneth Oliveira1, Robert E. Drew1, Kenneth L. Jones2,
Cris Hagen3, and Stacey Lance3

1Biology Department, University of Massachusetts Dartmouth,
285 Old Westport Road, North Dartmouth, MA 02747-2300, USA
2Georgia Genomics Facility, University of Georgia, Athens, GA 30602, USA
3Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA

Abstract: We isolated and characterized a total of 10 microsatellite loci from the Yellowtail flounder, Limanda ferruginea. Loci were screened in 24 individuals, 8 each, from the Georges Bank, Southern New England/Mid Atlantic, and Cape Cod/Gulf of Maine, management areas. The number of alleles per locus ranged from 5 to 23, observed heterozygosity ranged from 0.375 to 0.875, and the probability of identity values ranged from 0.102 to 0.505. These new loci will provide tools for examining existing management stock designations and for determining levels of gene flow between these stocks.

Keywords: Limanda, Yellowtail flounder, Microsatellite, PCR primers, SSR, STR

SREL Reprint #3171

Breton, J. S., K. Oliveira, R. E. Drew, K. L. Jones, C. Hagen, and S. Lance. 2011. Development and characterization of ten polymorphic microsatellite loci in the yellowtail flounder (Limanda ferruginea). Conservation Genetics Resources (2011) 3:369-371.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).