SREL Reprint #2932

Sixty polymorphic microsatellite markers for the oldfield mouse developed in Peromyscus polionotus and Peromyscus maniculatus

Lynne M. Mullen1, Rachel J. Hirschmann1, Kelly L. Prince2,3, Travis C. Glenn4, Michael J. Dewey2,3, and Hopi E. Hoekstra1

1Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA,
2Peromyscus Genetic Stock Center
3Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA,
4Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA

Abstract: We isolated and characterized 60 novel microsatellite markers from the closely related oldfield mouse (Peromyscus polionotus) and deer mouse (Peromyscus maniculatus) for studies of conservation, ecological, quantitative and population genetics. We assessed all 60 markers in a wild population of Peromyscus polionotus rhoadsi (N = 20) from central Florida and found an average of nine alleles per marker and an observed heterozygosity (HO) of 0.66 (range = 0.00–1.00). These polymorphic markers contribute to the growing number of genomic resources for Peromyscus, an emerging model system for ecological and evolutionary research.

Keywords: deer mouse, microsatellites, oldfield mouse, Peromyscus, Rodentia, simple sequence repeats

SREL Reprint #2932

Mullen, L. M., R. J. Hirschmann, K. L. Prince, T. C. Glenn, M. J. Dewey, and H. E. Hoekstra. 2006. Sixty polymorphic microsatellite markers for the oldfield mouse developed in Peromyscus polionotus and Peromyscus maniculatus. Molecular Ecology Notes 6:36-40.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).