SREL Reprint #3319

Development and characterization of 29 microsatellite markers for the sergeant major damselfish (Abudefduf saxatilis) using paired-end Illumina shotgun sequencing

Victor J. Piñeros1, Carla Gutiérrez-Rodríguez1, and Stacey L. Lance2

1Departamento de Biología Evolutiva, Instituto de Ecología A.C., 91070 Xalapa, Veracruz, Mexico
2Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA

Abstract: We isolated and characterized microsatellite loci for the coral reef fish Abudefduf saxatilis using Illumina paired-end-sequencing and tested 48 loci for amplification and polymorphism. We screened 29 polymorphic loci in individuals from Reserva de la Biósfera Banco Chinchorro (RBBC), Quintana Roo, Mexico to assess variability. Number of alleles per locus varied from 8 to 20, observed and expected heterozygosities from 0.458 to 1.00 and from 0.820 to 0.931, respectively. Some of the primers amplified individuals of four congeneric species. These markers will constitute invaluable tools for elucidating patterns of connectivity among populations of coral reef fishes, providing important information for the conservation of coral reefs.

Keywords: Connectivity, Coral reef fishes, Marine conservation, Next generation sequencing, Population genetics

SREL Reprint #3319

Piñeros, V. J., C. Gutiérrez-Rodríguez, and S. L. Lance. 2015. Development and characterization of 29 microsatellite markers for the sergeant major damselfish (Abudefduf saxatilis) using paired-end Illumina shotgun sequencing. Conservation Genetics Resources 7(1): 103-105.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).