SREL Reprint #3109

253 Novel polymorphic microsatellites for the saltwater crocodile (Crocodylus porosus)

Lee G. Miles1, Sally R. Isberg1,2, Chris Moran1, Cris Hagen3, and Travis C. Glenn3,4

1Faculty of Veterinary Science, University of Sydney, Room 513, RMC Gunn Building, Sydney, NSW 2006, Australia
2Porosus Pty Ltd, P.O. Box 86, Palmerston, NT 0831, Australia
3Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, SC 29802, USA
4Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA

Abstract: Genomic elucidation and mapping of novel organisms requires the generation of large genetic resources. In this study, 253 novel and polymorphic microsatellite loci were isolated and characterized for the saltwater crocodile (Crocodylus porosus) by constructing libraries enriched for microsatellite DNA. All markers were evaluated on animals obtained from Darwin Crocodile Farm in the Northern Territory, Australia, and are intended for future use in the construction of a genetic-linkage map for the saltwater crocodile. The 253 loci yielded an average of 4.12 alleles per locus, and those selected for mapping had an average polymorphic information content (PIC) of 0.425.

Keywords: Estuarine crocodile, Reptile, SSRs, Primers, Tetra-nucleotide repeats, Tri-nucleotide repeats, Di-nucleotide repeats, Enrichment

SREL Reprint #3109

Miles, L. G., S. R. Isberg, C. Moran, C. Hagen, and T. C. Glenn. 2009. 253 Novel polymorphic microsatellites for the saltwater crocodile (Crocodylus porosus). Conservation Genetics 10(2009): 963-980.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).