SREL Reprint #3120

Fifteen polymorphic microsatellite loci from Jamaican streamertail hummingbirds (Trochilus)

Stacey L. Lance1,2, Cris Hagen2, Travis C. Glenn1, Robb T. Brumfield3,4,
Katherine Faust Stryjewski3,4, and Gary R. Graves5

1Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA
2Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
3Museum of Natural Science, Louisiana State University, 119 Foster Hall, Baton Rouge, LA 70803, USA
4Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
5Department of Vertebrate Zoology MRC-116, National Museum of Natural History,
Smithsonian Institution, P.O. Box 37012, Washington, DC 20013-7012, USA

Abstract: We isolated and characterized 15 microsatellite loci from the endemic Jamaican streamertail hummingbird Trochilus polytmus. Loci were screened in 12 individuals of both T. polytmus and its sister species T. scitulus, also a Jamaican endemic. The number of alleles per locus ranged from 2 to 10, observed heterozygosity ranged from 0 to 1, and the probability of identity values ranged from 0.038 to 0.663. These new loci provide tools for characterizing the narrow hybrid zone between the two species.

Key words: Black-billed streamertail, Hummingbird, Jamaica, Microsatellite, PCR primers, SSR, STR, Red-billed streamertail, Trochilus polytmus, Trochilus scitulus

SREL Reprint #3120

Lance, S. L., C. Hagen, T. C. Glenn, R. T. Brumfield, K. F. Stryjewski, and G. R. Graves. 2009. Fifteen polymorphic microsatellite loci from Jamaican streamertail hummingbirds (Trochilus). Conservation Genetics 2009(10): 1195-1198.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).