SREL Reprint #3213

Development and Characterization of Microsatellite Markers for Polygonum cespitosum (Polygonaceae)

Silvia Matesanz1,2, Sonia E. Sultan1, Kenneth L. Jones3, Cris Hagen4, and Stacey L. Lance4

1Biology Department, Wesleyan University, Middletown, Connecticut 06459 USA
2Laboratorio Internacional de Cambio Global (LINC-Global), Museo Nacional de Ciencias Naturales,
CSIC, Madrid 28006 Spain
3Georgia Genomics Facility, University of Georgia, Athens, Georgia 30602 USA
4Savannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802 USA

Premise of the study: We isolated and characterized microsatellite markers in Polygonum cespitosum Blume, an herbaceous annual plant species introduced into North America from Asia that has recently become invasive.
Methods and Results: A total of 12 polymorphic and 3 monomorphic loci were screened in 1–2 individuals from each of 20 populations from the introduced and native range, for a total of 24 samples. The number of alleles per locus in the polymorphic loci ranged from 3 to 9, and expected heterozygosity ranged from 0.156 to 0.838.
Conclusions: These new loci will provide tools for examining genetic relatedness among introduced and native populations of this and other related species.

Keywords: microsatellite, PCR primers, Persicaria, Polygonum, SSR, STR.

SREL Reprint #3213

Matesanz, S., S. E. Sultan, K. L. Jones, C. Hagen, and S. L. Lance. 2011. Development and Characterization of Microsatellite Markers for Polygonum cespitosum (Polygonaceae). American Journal of Botany 98(7): e180-e182.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).