SREL Reprint #3085

Microsatellite markers isolated from polyploid wood-sorrel, Oxalis alpina (Oxalidaceae)

Olga V. Tsyusko1, Tracey D. Tuberville1, Maureen B. Peters1, Nicholas Crawford1,
Cris Hagen1, Stephen G. Weller2, Ann K. Sakai2, and Travis C. Glenn1

1Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, SC 29802, USA
2Department of Ecology and Evolutionary Biology, University of California-Irvine, Irvine, CA 92697, USA

Abstract: Twelve polymorphic microsatellite loci were isolated from polyploid alpine wood-sorrel, Oxalis alpina (Oxalidaceae), and optimized for future studies of its breeding system. The loci were screened for variability among 72 individuals from Pinos Altos, New Mexico. The primers amplified loci with allele number ranging from two to 17 per locus and with estimates of Nei’s genetic diversity varying from 0.10 to 0.99. These primers provide an opportunity to use polymorphic DNA markers to study the causes of breeding system variability in this species.

Keywords: breeding system, microsatellites, Oxalis alpina, PCR, polymorphism, polyploidy, primer

SREL Reprint #3085

Tsyusko, O. V., T. D. Tuberville, M. B. Peters, N. G. Crawford, C. Hagen, S. G. Weller, A. K. Sakai and T. C. Glenn. 2007. Microsatellite markers isolated from polyploid wood-sorrel, Oxalis alpina (Oxalidaceae). Molecular Ecology Notes 2007(7): 1284-1286.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).