SREL Reprint #2711

Seven polymorphic microsatellite DNA loci from the red-spotted newt (Notophthalmus viridescens)

Dean A. Croshaw1,2 and Travis C. Glenn1,3

1Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
2Department of Biological Sciences, University of New Orleans, New Orleans, LA 70148, USA
3Department of Biological Sciences, University of South Carolina, Columbia, SC29208, USA

Abstract: We describe polymerase chain reaction (PCR) primers and amplification conditions for seven microsatellite DNA loci isolated from the red-spotted newt (Notophthalmus viridescens). Primers were tested on 16 individuals from two populations on the Savannah River Site in Aiken County, South Carolina. We detected six to 10 alleles per locus and an overall observed heterozygosity range of 0.31-0.81. Despite low heterozygosity at two of the seven loci, the high polymorphic information contents (from 0.54 to 0.85) of these markers render them useful for future studies of the behavioural and population ecology of this common salamander.

Keywords: microsatellites, newts, Notophthalmus viridescens, PCR primers, SSR, STR

SREL Reprint #2711

Croshaw, D. A. and T. C. Glenn. 2003. Seven polymorphic microsatellite DNA loci from the red-spotted newt (Notophthalmus viridescens). Molecular Ecology Notes 3: 514-516.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).