SREL Reprint #3153

Fifteen microsatellite loci for the decollate snail, Rumina decollata

Stacey L. Lance1, Kenneth L. Jones2, Cris Hagen1, Kurt Jordaens3,4, Thierry Backeljau4,5, and Vanya Prévot5,6

1Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA
2Georgia Genomics Facility, University of Georgia, Athens, GA 30602, USA
3Royal Museum for Central Africa, Leuvensesteenweg 13, 3080 Tervuren, Belgium
4Evolutionary Ecology Group, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium
5Royal Belgian Institute of Natural Sciences, Vautierstraat 29, 1000 Brussels, Belgium
6Evolutionary Biology and Ecology, Université Libre de Bruxelles (CP 160/12),
Av. F.D. Roosevelt, 50, 1050 Brussels, Belgium

Abstract: We characterized 15 microsatellite loci from the decollate snail, Rumina decollata. Loci were screened in 21 individuals and several individuals of the congener Rumina saharica. There was ample allelic diversity (6–12 alleles per locus) but observed heterozygosity values were extremely low (0–0.421). This was expected given the high self-fertilization rate in this species. Ten of the 15 loci were successfully amplified in R. saharica. These loci provide tools for examining the population genetics and taxomomic boundaries in R. decollata and its allies.

Keywords: Rumina, Land snail, Microsatellite, PCR primers, SSR, STR

SREL Reprint #3153

Lance, S. L., K. L. Jones, C. Hagen, K. Jordaens, T. Backeljau, and V. Prévot. 2010. Fifteen microsatellite loci for the decollate snail, Rumina decollata. Conservation Genetics Resources 2010(2): 287-289.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).