SREL Reprint #3072
Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards
Nicholas G. Crawford1,3, Jaime Zaldívar-Rae2, Cris Hagen3, Amanda Schable3,
Erica Bree Rosenblum4,5, Jeff A. Graves6, Tod W. Reeder1, Michael G. Ritchie6, and
Travis C. Glenn3
1Department of Biology, San Diego State University, San Diego, CA 92182–4614, USA
2Laboratorio de Conducta Animal, Instituto de Ecología, Universidad Nacional Autónoma de México,
A.P. 70–275, C.P. 04510, México, D.F. Mexico
3Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
4Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
5Department of Genome Sciences, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
6Environmental and Evolutionary Biology, University of St. Andrews, KY16 9TS, UK
Abstract: We describe polymerase chain reaction primers and amplification conditions for 13 microsatellite DNA loci isolated from two bisexual species of whiptail lizards Aspidoscelis costata huico and Aspidoscelis inornata. Primers were tested on either 16 or 48 individuals of A. c. huico and/or 26 individuals of A. inornata. Ten of the 13 primers were also tested against a panel of 31 additional whiptail taxa. We detected three to nine alleles per locus in A. c. huico and four to 19 alleles per locus in A. inornata, with observed heterozygosity ranging from 0.60 to 0.87 and from 0.15 to 1.00, respectively. These primers will be an important resource for surveys of genetic variation in these lizards.
Keywords: Ameiva, Aspidoscelis, Cnemidophorus, lizard, microsatellite, PCR primers, SSR, STR, teiid,
whiptail
SREL Reprint #3072
Crawford, N. G., J. Zaldívar-Rae, C. Hagen, A. Schable, E. B. Rosenblum, J. A. Graves, T. W. Reeder, M. G. Ritchie and T. C. Glenn. 2008. Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards. Molecular Ecology Resources 2008(8): 219-223.
This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).