SREL Reprint #3127

Development and characterization of twelve polymorphic microsatellite loci in the threatened Red Hills salamander, Phaeognathus hubrichti

Stacey L. Lance1,2, Cris Hagen2, Travis C. Glenn1, Joseph J. Apodaca3, and Leslie J. Rissler3

1Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA
2Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
3Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487, USA

Abstract: We isolated and characterized 12 microsatellite loci from the endangered Red Hills salamander, Phaeognathus hubrichti. Loci were screened in 24 individuals of P. hubrichti. The number of alleles per locus ranged from 2 to 16, observed heterozygosity ranged from 0.130 to 0.750, and the probability of identity values ranged from 0.012 to 0.778. These new loci provide tools for examining the population genetics of this federally threatened salamander.

Key words: Red Hills salamander, Phaeognathus, Microsatellite, PCR primers, SSR, STR

SREL Reprint #3127

Lance, S. L., C. Hagen, T. C. Glenn, J. J. Apodaca, and L. J. Rissler. 2009. Development and characterization of twelve polymorphic microsatellite loci in the threatened Red Hills salamander, Phaeognathus hubrichti. Conservation Genetics 10: 1919-1921.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).