SREL Reprint #3332
Characterization of microsatellite loci for an Australian epiphytic orchid, Dendrobium calamiforme, using Illumina sequencing
Dorset W. Trapnell1, Rochelle R. Beasley2, Stacey L. Lance2, Ashley R. Field3,4, and Kenneth L. Jones5
1Plant Biology Department, University of Georgia, Athens, Georgia 30602 USA
2Savannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802 USA
3Queensland Herbarium, Department of Science, Information Technology and Innovation,
Toowong, Queensland 4066, Australia
4Australian Tropical Herbarium, James Cook University, Smithfield, Queensland 4878, Australia
5Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine,
Aurora, Colorado 80045 USA
• Premise of the study: Microsatellite loci were developed for the epiphytic pencil orchid Dendrobium calamiforme for population genetic and phylogeographic investigation of this Australian taxon.
• Methods and Results: Nineteen microsatellite loci were identified from an Illumina paired-end shotgun library of D. calamiforme. Polymorphism and genetic diversity were assessed in 24 individuals from five populations separated by a maximum distance of ~80 km. All loci were polymorphic with two to 14 alleles per locus, expected heterozygosity ranging from 0.486 to 0.902, and probability of identity values ranging from 0.018 to 0.380.
• Conclusions: These novel markers will serve as valuable tools for investigation of levels of genetic diversity as well as patterns of gene flow, genetic structure, and phylogeographic history.
Keywords: Dendrobium calamiforme; Dockrillia calamiformis; genetic diversity; Orchidaceae; phylogeography; simple sequence repeat (SSR) markers
SREL Reprint #3332
Trapnell, D. W., R. R. Beasley, S. L. Lance, A. R. Field, and K. L. Jones. 2015. Characterization of microsatellite loci for an Australian epiphytic orchid, Dendrobium calamiforme, using Illumina sequencing. Applications in Plant Sciences 3(6): 1-4.
This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).