SREL Reprint #3254

Development and characterization of twenty-three microsatellite markers for the freshwater minnow Santa Ana speckled dace (Rhinichthys osculus spp., Cyprinidae) using paired-end Illumina shotgun sequencing

Schyler O. Nunziata1, Stacey L. Lance1, Kenneth L. Jones2, Stacey A. Nerkowski3, and Anthony E. Metcalf3

1Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA
2Department of Biochemistry and Molecular Genetics, School of Medicine,
University of Colorado, Aurora, CO 80045, USA
3Department of Biology, California State University, San Bernardino, San Bernardino, CA 92407, USA

Abstract: We isolated and characterized a total of 23 microsatellite loci from the Santa Ana speckled dace, Rhinichthys osculus spp., a freshwater minnow restricted to southern California. Loci were screened in 24 individuals from five watersheds. The number of alleles per locus ranged from 7 to 25, observed heterozygosity ranged from 0.409 to 0.875, and the probability of identity values ranged from 0.005 to 0.081. These new loci will provide tools for examining taxonomic status, population structure, and management strategies.

Keywords: Rhinichthys osculus, Illumina, Microsatellite, PAL_Finder, PCR primers, SSR, STR

SREL Reprint #3254

Nunziata, S. O., S. L. Lance, K. L. Jones, S. A. Nerkowski, and A. E. Metcalf. 2013. Development and characterization of twenty-three microsatellite markers for the freshwater minnow Santa Ana speckled dace (Rhinichthys osculus spp., Cyprinidae) using paired-end Illumina shotgun sequencing. Conservation Genetic Resources 5(1):145-148.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).