SREL Reprint #2612

Cross-species amplification among peromyscines of new microsatellite DNA loci from the oldfield mouse (Peromyscus polionotus subgriseus)

Kelly L. Prince1,2, Travis C. Glenn2,3, and Michael J. Dewey1,2

1Peromyscus Genetic Stock Center, University of South Carolina, Columbia, SC 29208, USA
2Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA
3Savannah River Ecology Laboratory, P.O. Drawer E, Aiken, SC 29802, USA

Abstract: We describe polymerase chain reaction (PCR) primers and conditions to amplify 11 microsatellite DNA loci isolated from the oldfield mouse (Peromyscus polionotus subgriseus). These were tested for amplification using nine species and subspecies maintained at the Peromyscus Genetic Stock Center, with an average success rate of 65% and two loci amplifying in all species. Polymorphism was tested within the P. polionotus subgriseus and the recently obtained P. maniculatus sonorensis colonies. P. p. subgriseus had modest numbers of alleles per locus (1-4), whereas P. m. sonorensis had many alleles per locus (5-10) and high expected heterozygosities (0.625-0.878).

Keywords: dinucleotide repeats, PCR, Peromyscus, primer, Rodentia, SSR

SREL Reprint #2612

Prince, K. L., T. C. Glenn, and M. J. Dewey. 2002. Cross-species amplification among peromyscines of new microsatellite DNA loci from the oldfield mouse (Peromyscus polionotus subgriseus). Molecular Ecology Notes 2:133-136.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).