SREL Reprint #3298

Development and characterization of twenty-five microsatellite markers for the longnose dace (Cyprinidae: Rhinichthys) using paired-end Illumina shotgun sequencing

Rochelle Beasley1, Stacey L. Lance1, Jennifer A. Ruskey2, and Eric B. Taylor2

1Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA
2Department of Zoology, Biodiversity Research Centre and Beaty Biodiversity Museum,
University of British Columbia, 6270 University Blvd., Vancouver, BC V6T 1Z4, Canada

Abstract: We isolated and characterized a total of 48 microsatellite loci in Rhinichthys cataractae a widely distributed freshwater fish that may contain several cryptic species. Loci were screened in 24 individuals from several areas of British Columbia, Canada. The number of alleles per locus ranged from 5 to 24, observed heterozygosity ranged from 0.250 to 0.940, and the probability of identity ranged from 0.006 to 0.453. These new loci are being used for conducting investigations into the genetic structure, diversity, and speciation in populations of this exceptionally broadly-distributed North American freshwater fish.

Keywords: Rhinichthys, Microsatellite, PAL_FINDER, PCR primers, SSR

SREL Reprint #3298

Beasley, R. R., S. L. Lance, J. A. Ruskey, and E. B. Taylor. 2014. Development and characterization of twenty-five microsatellite markers for the longnose dace (Cyprinidae: Rhinichthys) using paired-end Illumina shotgun sequencing. Conservation Genetic Resources 6: 1011-1013.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).