SREL Reprint #2782

Tetranucleotide and dinucleotide microsatellite loci from the northern bobwhite (Colinus virginianus)

Nancy A. Schable1,3, Brant C. Faircloth2, William E. Palmer4, John P. Carroll2, L. Wes Burger5,
Leonard A. Brennan6, Cris Hagen1, and Travis C. Glenn1,3

1Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, SC 29802, USA
2D. B. Warnell School of Forest Resources, University of Georgia, Athens, GA 30602, USA
3Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA
4Tall Timbers Research Station, 13093 Henry Beadel Drive, Tallahassee, FL 32312, USA
5Department of Wildlife and Fisheries, Box 9690, Mississippi State University, Mississippi State,
MS 39762-9690, USA
6Richard M. Kleberg Jr Center for Quail Research, Texas A & M University-Kingsville, 700 University Boulevard, MSC 218, Kingsville, TX 78363-8202, USA

Abstract: We describe polymerase chain reaction (PCR) primers and conditions to amplify eight dinucleotide, one trinucleotide and 14 tetranucleotide microsatellite DNA loci isolated from the northern bobwhite (Colinus virginianus). The PCR primers were tested on 16 individuals collected from a population located within the Red Hills region of south Georgia and north Florida. The 23 primer pairs developed in this study yielded an average of 6.5 alleles per locus (range 2-11), an average observed heterozygosity of 0.47 (range 0.06-0.94) and average polymorphic information content of 0.60 (range 0.06-0.85).

Keywords: Colinus virginianus, Galliformes, microsatellites, PCR, primer, quail, SSR

SREL Reprint #2782

Schable, N. A., B. C. Faircloth, W. E. Palmer, J. P. Carroll, L. W. Burger, L. A. Brennan, C. Hagen, and T. C. Glenn. 2004. Tetranucleotide and dinucleotide microsatellite loci from the northern bobwhite (Colinus virginianus). Molecular Ecology 4:415-419.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).