SREL Reprint #3125

Development and characterization of nineteen polymorphic microsatellite loci from seaside alder, Alnus maritima

Stacey L. Lance1,2, Kenneth L. Jones1, Cris Hagen2, Travis C. Glenn1, J. Matthew Jones3, and J. Phil Gibson3,4

1Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA
2Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
3Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA
4Department of Zoology, University of Oklahoma, Norman, OK 73019, USA

Abstract: We isolated and characterized 19 microsatellite loci from the endangered seaside alder, Alnus maritima. Loci were screened in 24 individuals of A. maritima and four individuals of its congener the hazel alder, A. serrulata. The number of alleles per locus ranged from 2 to 6, observed heterozygosity ranged from 0 to 0.952, and the probability of identity values ranged from 0.126 to 0.850. These new loci provide tools for characterizing the population genetics of this rare tree.

Key words: Seaside alder, Hazel alder, Microsatellite, PCR primers, SSR, STR, Alnus maritima, Alnus serrulata

SREL Reprint #3125

Lance, S. L., K. L. Jones, C. Hagen, T. C. Glenn, J. M. Jones, and J. P. Gibson. 2009. Development and characterization of nineteen polymorphic microsatellite loci from seaside alder, Alnus maritima. Conservation Genetics 10: 1907-1910.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).